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Sr Research Assistant – (PCR, Western Blot)

MD Anderson Cancer Center

This is a Full-time position in Houston, TX posted May 19, 2021.

The mission of The University of Texas M.

D.

Anderson Cancer Center is to eliminate cancer in Texas, the nation, and the world through outstanding programs that integrate patient care, research and prevention, and through education for undergraduate and graduate students, trainees, professionals, employees and the public.The primary purpose of the Sr.

Research Assistant position is to exercise considerable imagination, initiative and inventiveness in planning and executing the details of experiments and in evaluating and interpreting the results.

Responsible for major participation in the development and revision of techniques, assembles and operates laboratory apparatus and equipment.

Collaborates or assists with supervisor and senior research staff with preparation of reports and grant requests.

Supervises technicians in their work and assists them as assigned or delegated.**Research and Development**Research and Development in the areas of Cancer Biology, Molecular Biology, Immunology, Virology, Cell Biology, and Biochemistry.
• DNA & RNA isolation and plasmid purification
• Transfection
• Western blotting, PCR and genotyping
• Cell growth, proliferation and apoptosis assays
• MTT and colony formation assays
• FACS analysis
• Basic immunohistochemistry assays
• Animal studies (mice) including subcutaneous implantation of cancer cells, intravenous and intraperitoneal injection of drugs and measurement of tumor volumes
• Radiation usage**Cell Culture and Record Keepin** gThe ability to perform general cell culture procedures using sterile technique, which would involve the growth and maintenance of various cell lines.

Maintain Cell Culture Cell freezing, seeding, passage, and viability/growth analysis/cell death.

Conduct molecular analysis in tissue culture cells.Record keeping and data maintenance to meet supervisor, department / institute directives.

Records experimental data by manually writing in a notebook or enter into a computer database.

Collects and accurately calculate experimental data under the guidance of supervisor.

Prepares graphs and charts by using computer.Collaborates or assists with supervisor and senior research staff with preparation of reports and grant requests.

Supervises technicians in their work and assists them as assigned or delegated.General Lab DutiesAssists with general lab functions as directed by lab supervisor.

Maintains condition of -80C freezers in main laboratory.

Coordinate with department lab coats receiving and billing.

Participate in equipment validation.

Participate in an “as needed basis” on projects assigned by research supervisor.Other duties as assignedEducation Required:Bachelor’s degree in one of the natural sciences or related field.Education PreferredMaster’s degreeExperience Required:Three years of experience in scientific or experimental research work.

With preferred degree, one year of required experienceExperience Preferred
– Cell culture experience
– Basic molecular biology/cell biology experience (PCR, Western blot)
– Mouse handling experienceIt is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law.

danderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.htmlAdditional Information+ Requisition ID: 138645+ Employment Status: Full-Time+ Employee Status: Regular+ FLSA: exempt and not eligible for overtime pay+ Work Week: Days+ Fund Type: Soft+ Pivotal Position: Yes+ Minimum Salary: US Dollar (USD) 39,200+ Midpoint Salary: US Dollar (USD) 49,000+ Maximum Salary : US Dollar (USD) 58,800+ Science Jobs: Yes

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